Chemical analysis
Determination of acidity: take Fungal Chitosan Manufacturer the goods 0.5 g, 50 ml of water dissolves, determined in accordance with the law, the pH value should be 5.6 ~ 6.1.
Light transmittance of the solution: take the goods of 0.5 g, 20 ml of water dissolves, as spectrophotometric method, the determination of light transmittance at the wavelength of 430 nm, shall not be less than 98.0%.
Chloride test: take the goods 0.30 g, inspection in accordance with the law, with the standard control fluid is made of sodium chloride solution of 6.0 ml, shall not be thicker (0.02%). Sulfate test: take the goods 1.0 g, inspection in accordance with the law, and the standard potassium sulfate solution control fluid is made of 2.0 ml, shall not (0.02%).
Ammonium salt test: take the goods 0.10 g, inspection in accordance with the law, with the standard control fluid is made of 2.0 ml ammonium chloride solution, shall not be deeper (0.02%). Determination of other s Adenosyl methionine: take the goods, add water to make each 1 ml contains 10 mg of solution, according to the thin layer chromatography (TLC) test, drain the solution above 5 mu l, points on the silica Quercetin Dihydrate Manufacturer G thin layer plate, n-butyl alcohol - glacial acetic acid and water (4:1:5) as developing agent, unfolds, dry, dry in 90 ℃ for 10 minutes, spray with ninhydrin acetone solution (0.5-100), heating at 90 ℃ for 10 minutes, immediately review, should only show a purple spots.
Dry weight loss: take the goods, 3 hours at 105 ℃ dry, loss of weight reduction shall not be over 0.2%. On ignition residue may not be over 0.1%. Iron to take the goods of 1.0 g, inspection in accordance with the law, with the standard control fluid is made of iron solution 1.5 ml, shall not be deeper (0.0015%). Take the goods 0.50 g of heavy metals, 23 ml water dissolves, add acetate buffer (PH3.5) 2 ml, inspection in accordance Berberine Hydrochloride Factory with the law, containing heavy metals shall not be ten over one million. Take the goods 2.0 g arsenic salt, 23 ml water dissolves, add 5 ml of hydrochloric acid, inspection in accordance with the law, shall comply with regulations (0.0001%).
Pyrogen determination: take the goods, and made each 1 ml contains 20 mg of sodium chloride injection solution, inspection in accordance with the law and dose per 1 kg weight on rabbit injected 10 ml, should comply with the rules (for injection).
Content determination
The method name: methionine API, the determination of methionine, potentiometric titration method
Application: apply the methods using titration method to determine methionine, sense of methionine content API. The method is suitable for methionine API.
Method principle: test samples with Thyroid Powder Factory anhydrous formic acid and glacial acetic acid dissolves, and potentiometric titration with perchloric acid titration fluid, and use the result of the titration blank test correction, according to the titrant usage, calculate the content of methionine.